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Animal cell culture : a practical approach / ed. R. I. Freshney

Secondary Author Freshney, R. Ian Country Reino Unido. Edition 2nd ed Publication Oxford : IRL Press, imp. 1995 Description XIX, 329 p. : il. ; 24 cm Series The practical approach series , 104) ISBN 0-19-963213-8 CDU 577.1
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Item type Current location Call number Status Date due Barcode Item holds Course reserves
Monografia Biblioteca Geral da Universidade do Minho
BGUM 577.1 - A Available 197523

Mestrado em Biotecnologia Riscos e Recursos Biológicos 2º semestre

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Enhanced descriptions from Syndetics:

Animal Cell Culture: A Practical Approach has sold over 10,000 copies since its publication in 1986, and remains one of the most popular titles in the series. This new edition takes account of the progress that has been made in the field. Although the basic principles remain the same,significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetricindicator of viability has made a considerable impact on cytotoxity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presentingtechniques in a readily accessible form, with detailed protocos given throughout. This volume will be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.

Table of contents provided by Syndetics

  • 1 Introduction to Basic Principles
  • 2 Towards Serum-free, Chemically Defined Media for Mammalian Cell Culturev
  • 3 Scaling-up of Animal Cell Cultures
  • 4 Cell Line Preservation and Characterization
  • 5 Separation of Viable Cells by Centrifugal Elutriation
  • 6 Flow Cytometry
  • 7 Organ Culture
  • 8 Cytotoxicity and Viability Assays
  • 9 In Situ Hybridization

Author notes provided by Syndetics

R. I. Freshney, CRC Department of Medical Oncology, University of Glasgow.

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